Optical & Atomic Force Microscopy Facilities

A significant amount of Biophysics research and Optics uses optical and AFM microscopes. The department houses several high-grade research microscopes with various options for viewing cells or other specimens including:

A. Optical Microscopes

1. Nikon Eclipse E600FN upright with DIC, fluorescence, and brightfield capabilities.
2. Olympus IX-71 inverted microscope with brightfield, dark field, and fluorescence capabilities, as well as optical tweezers.
3. Olympus BM60 metallurgical microscope for viewing non transmissive samples.
4. Nikon inverted microscope – with fluorescence, DIC, a low-noise scientific CMOS camera, environmental chamber (T, CO₂), computerized shutter.
5. Zeiss Axiovert 200 inverted microscope with brightfield, fluorescence and with a low-noise Hammamatsu emCCD camera or Zeiss AxioCam MRm camera.
6. Zeiss Observer D inverted microscope with fluorescence, DIC, and with an environmental chamber.
7. Zeiss LSM 710 Laser scanning confocal microscope within the Microscope Imaging Core Facility and Confocal Microscopy Center
8. Zeiss LSM 880 with Airyscan – laser scanning confocal miscroscope wthin the Microscope Imaging Core Facility and Confocal Microscopy Center – located at Wake Downtown.

B. Atomic Force Microscopes

1. Two Topometrix Explorer AFMs (Veeco), which can be controlled (haptic and 3D visual) by two nanoManipulators (3^rd Tech)
2. Nanoscope III Atomic Force Microscope (Veeco).
3. Asylum MFP-3D Bio AFM combined with an Olympus IX73 inverted optical microscope.

Fig. caption: The Olympus IX73 inverted optical microscope with the Asylum MFP-3D Bio Atomic Force Microscope on top of stage in the center of the photo.